Stefan Ruhl, Ph.D. Department of Oral Biology School of Dental Medicine University at Buffalo 3435 Main Street Foster Hall, Room 213A Phone: (716) 829-6073 E-mail:  shruhl@buffalo.edu

Our laboratory is interested in the proteins of human saliva that are recognized by microbial adhesins. It is our long-term goal to better understand the modulating role of salivary glycoproteins in supporting tissue tropism of a benign commensal oral microflora to the human oral cavity and in host defense against pathogenic microorganisms.
A detailed description of our current research activities can be found on the following website: http://www.sdm.buffalo.edu/oralbiology/home.asp?f=shruhl

Select Publications
Bürgers R, Schneider-Brachert W, Reischl U, Behr A, Hiller K-A, Lehn N, Schmalz G, Ruhl S  (2008)  Helicobacter pylori in human oral cavity and stomach.  Eur. J. Oral Sci. 116:297-304.

Müller R, Ruhl S, Hiller K-A, Schmalz G, Schweikl H  (2007)  Adhesion of eukaryotic cells and Staphylococcus aureus to silicon model surfaces. J. Biomed. Mater. Res. A. 84A: 817-827.

Müller R, Gröger G, Hiller K-A, Schmalz G, Ruhl S  (2007)  Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria. Appl. Environ. Microbiol. 73: 2653-2660.

Müller R, Hiller K-A, Schmalz G, Ruhl S  (2006)  Chemiluminescence-based detection and comparison of protein amounts adsorbed on differently modified silica surfaces. Anal. Biochem. 359: 194-202.

Aspholm M, Kalia A, Ruhl S, Schedin S, Arnqvist A, Sjöström R, Gerhard M, Semino-Mora C, Dubois A, Unemo M, Danielsson D, Teneberg S, Kon W, Berg D, Borén T (2006) Helicobacter pylori adhesion to carbohydrates.  Meth. Enzymol. 417: 293-339.

Walz A, Wattenberg A, Meyer HE, Schmalz G, Blüggel M, Ruhl S  (2006)  Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis.  Proteomics 6: 1631-1639.

Walz A, Odenbreit S, Mahdavi J, Borén T, Ruhl S   (2005)  Identification and characterization of binding properties of Helicobacter pylori by glycoconjugate arrays.  Glycobiology 15: 700-708.

Ruhl S, Rayment SA, Schmalz G, Hiller K-A and Troxler RF   (2005)  Proteins in whole saliva during the first year of infancy.  J. Dent. Res. 84: 29-34.

Ruhl S, Sandberg AL and Cisar JO   (2004)  Salivary receptors for the proline rich protein-binding and lectin-like adhesins of oral actinomyces and streptococci.  J. Dent. Res. 83: 505-510.

Ruhl S, Hamberger S, Betz R, Sukkar T, Schmalz G, Seymour RA, Hiller K-A and Thomason JM   (2004)  Salivary proteins and cytokines in drug-induced gingival overgrowth.  J. Dent. Res. 83: 322-326.

Takahashi Y, Ruhl S, Yoon J-W, Sandberg AL and Cisar JO   (2002)  Adhesion of viridans group streptococci to sialic acid-, galactose- and N-acetylgalactosamine-containing receptors.  Oral Microbiol. Immunol. 17: 257-262.

Ruhl S, Cisar JO, Sandberg AL   (2000)  Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii.  Infect. Immun.  68: 6346-6354.

Takahashi Y, Sandberg AL, Ruhl S, Muller J, and Cisar JO   (1997)   A specific cell surface antigen of Streptococcus gordonii is associated with bacterial hemagglutination and adhesion to a2-3-linked sialic acid-containing receptors.  Infect. Immun. 65: 5042-5051.

Ruhl S, Sandberg AL, Cole MF, and Cisar JO  (1996)    Recognition of IgA1 by oral actinomyces and streptococcal lectins. Infect. Immun. 64: 5421-5424.

Sandberg AL, Ruhl S, Joralmon RA, Brennan MJ, Sutphin MJ, and Cisar JO  (1995)   Putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the Actinomyces naeslundii WVU45 lectin.  Infect. Immun. 63: 2625-2631.

Ruhl S, Feldman GM, Akahane K, and Pluznik DH  (1993)   Interferon-g antagonizes interleukin-6-induced expression of interleukin-4 receptors in murine myeloid cells by a transcriptional mechanism.  Blood 82: 2641-2648.

Ruhl S, Pluznik DH, and Feldman GM  (1993)   Soluble interleukin-4 receptor production by murine myeloid progenitor cells: induction by interleukin-6 and interferon-g.  Cytokine 5: 144-149.

Ruhl S and Pluznik DH  (1993)   Dissociation of early and late markers of murine myeloid differentiation by interferon-gamma and interleukin-6.  J. Cell. Physiol. 155: 130-138.

Ruhl S, Schwabe M, and Pluznik DH  (1992)   Interleukin-1 augments the expression of interleukin-2 receptors on differentiating myeloid cells.  Exp. Hematol. 20: 1208-1215.

Ruhl S, Begley CG, Bickel M, and Pluznik DH  (1992)   Transient expression of the IL-2 receptor a-chain in IL-6-induced myeloid cells is regulated by autocrine production of prostaglandin E2.  Exp. Hematol. 20:  619-625.

Feldman GM, Ruhl S, Bickel M, Finbloom D, and Pluznik DH  (1991)   Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6.  Blood 78:  1678-1684.

Bickel M, Dveksler G, Dieffenbach CW, Ruhl S, Midura SB, and Pluznik DH  (1990)   Induction of interferon-b and 2'5'-oligoadenylate synthetase mRNA by interleukin-6 during differentiation of murine myeloid cells.  Cytokine 2: 1-9.

Najar HM, Ruhl S, Bru-Capdeville AC, and Peters JH  (1990)   Adenosine and its derivates control human monocyte differentiation into highly accessory cells versus macrophages.  J. Leuk. Biol. 47: 429-439.

Peters JH, Ruhl S, and Friedrichs D  (1987)   Veiled accessory cells deduced from monocytes. 
Immunobiol.  176: 154-166.