University at Buffalo
The Witebsky Center

The Witebsky Center
University at Buffalo
Bacteriology hostmicrobe immunology parasitology virology bioinformatics mycology
The Witebsky Center The Witebsky Center
Stefan Ruhl, Ph.D.

Stefan Ruhl, Ph.D.
Department of Oral Biology
School of Dental Medicine
University at Buffalo
3435 Main Street
Foster Hall, Room 213A
Phone: (716) 829-6073

Our laboratory is interested in the proteins of human saliva that are recognized by microbial adhesins. It is our long-term goal to better understand the modulating role of salivary glycoproteins in supporting tissue tropism of a benign commensal oral microflora to the human oral cavity and in host defense against pathogenic microorganisms.
A detailed description of our current research activities can be found on the following website:

Select Publications
Bürgers R, Schneider-Brachert W, Reischl U, Behr A, Hiller K-A, Lehn N, Schmalz G, Ruhl S  (2008)  Helicobacter pylori in human oral cavity and stomach.  Eur. J. Oral Sci. 116:297-304.

Müller R, Ruhl S, Hiller K-A, Schmalz G, Schweikl H  (2007)  Adhesion of eukaryotic cells and Staphylococcus aureus to silicon model surfaces. J. Biomed. Mater. Res. A. 84A: 817-827.

Müller R, Gröger G, Hiller K-A, Schmalz G, Ruhl S  (2007)  Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria. Appl. Environ. Microbiol. 73: 2653-2660.

Müller R, Hiller K-A, Schmalz G, Ruhl S  (2006)  Chemiluminescence-based detection and comparison of protein amounts adsorbed on differently modified silica surfaces. Anal. Biochem. 359: 194-202.

Aspholm M, Kalia A, Ruhl S, Schedin S, Arnqvist A, Sjöström R, Gerhard M, Semino-Mora C, Dubois A, Unemo M, Danielsson D, Teneberg S, Kon W, Berg D, Borén T (2006) Helicobacter pylori adhesion to carbohydrates.  Meth. Enzymol. 417: 293-339.

Walz A, Wattenberg A, Meyer HE, Schmalz G, Blüggel M, Ruhl S  (2006)  Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis.  Proteomics 6: 1631-1639.

Walz A, Odenbreit S, Mahdavi J, Borén T, Ruhl S   (2005)  Identification and characterization of binding properties of Helicobacter pylori by glycoconjugate arrays.  Glycobiology 15: 700-708.

Ruhl S, Rayment SA, Schmalz G, Hiller K-A and Troxler RF   (2005)  Proteins in whole saliva during the first year of infancy.  J. Dent. Res. 84: 29-34.

Ruhl S, Sandberg AL and Cisar JO   (2004)  Salivary receptors for the proline rich protein-binding and lectin-like adhesins of oral actinomyces and streptococci.  J. Dent. Res. 83: 505-510.

Ruhl S, Hamberger S, Betz R, Sukkar T, Schmalz G, Seymour RA, Hiller K-A and Thomason JM   (2004)  Salivary proteins and cytokines in drug-induced gingival overgrowth.  J. Dent. Res. 83: 322-326.

Takahashi Y, Ruhl S, Yoon J-W, Sandberg AL and Cisar JO   (2002)  Adhesion of viridans group streptococci to sialic acid-, galactose- and N-acetylgalactosamine-containing receptors.  Oral Microbiol. Immunol. 17: 257-262.

Ruhl S, Cisar JO, Sandberg AL   (2000)  Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii.  Infect. Immun.  68: 6346-6354.

Takahashi Y, Sandberg AL, Ruhl S, Muller J, and Cisar JO   (1997)   A specific cell surface antigen of Streptococcus gordonii is associated with bacterial hemagglutination and adhesion to a2-3-linked sialic acid-containing receptors.  Infect. Immun. 65: 5042-5051.

Ruhl S, Sandberg AL, Cole MF, and Cisar JO  (1996)    Recognition of IgA1 by oral actinomyces and streptococcal lectins. Infect. Immun. 64: 5421-5424.

Sandberg AL, Ruhl S, Joralmon RA, Brennan MJ, Sutphin MJ, and Cisar JO  (1995)   Putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the Actinomyces naeslundii WVU45 lectin.  Infect. Immun. 63: 2625-2631.

Ruhl S, Feldman GM, Akahane K, and Pluznik DH  (1993)   Interferon-g antagonizes interleukin-6-induced expression of interleukin-4 receptors in murine myeloid cells by a transcriptional mechanism.  Blood 82: 2641-2648.

Ruhl S, Pluznik DH, and Feldman GM  (1993)   Soluble interleukin-4 receptor production by murine myeloid progenitor cells: induction by interleukin-6 and interferon-g.  Cytokine 5: 144-149.

Ruhl S and Pluznik DH  (1993)   Dissociation of early and late markers of murine myeloid differentiation by interferon-gamma and interleukin-6.  J. Cell. Physiol. 155: 130-138.

Ruhl S, Schwabe M, and Pluznik DH  (1992)   Interleukin-1 augments the expression of interleukin-2 receptors on differentiating myeloid cells.  Exp. Hematol. 20: 1208-1215.

Ruhl S, Begley CG, Bickel M, and Pluznik DH  (1992)   Transient expression of the IL-2 receptor a-chain in IL-6-induced myeloid cells is regulated by autocrine production of prostaglandin E2.  Exp. Hematol. 20:  619-625.

Feldman GM, Ruhl S, Bickel M, Finbloom D, and Pluznik DH  (1991)   Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6.  Blood 78:  1678-1684.

Bickel M, Dveksler G, Dieffenbach CW, Ruhl S, Midura SB, and Pluznik DH  (1990)   Induction of interferon-b and 2'5'-oligoadenylate synthetase mRNA by interleukin-6 during differentiation of murine myeloid cells.  Cytokine 2: 1-9.

Najar HM, Ruhl S, Bru-Capdeville AC, and Peters JH  (1990)   Adenosine and its derivates control human monocyte differentiation into highly accessory cells versus macrophages.  J. Leuk. Biol. 47: 429-439.

Peters JH, Ruhl S, and Friedrichs D  (1987)   Veiled accessory cells deduced from monocytes. 
Immunobiol.  176: 154-166.