Department of Biochemistry
School of Medicine & Biomedical Sciences
Recent Publications

Kenneth M. Blumenthal, Ph.D.

Edgerton, GB, Blumenthal, KM, and Hanck, DA (2008) "Evidence for multiple effects of ProTx-II on activation gating in Nav1.5," Toxicon 52, 489-500 [PDF]

Page, B., Young, R., Iyer, V., Suzuki, G., Lis, M., Blumenthal, K.M., and Canty, J.M. (2007) “Persistent regional downregulation in mitochondrial enzymes and upregulation of stress proteins in swine with chronic hibernating myocardium,” Circ. Res. In press.

Coling, D., Ding, D., Lis, M., Young, R., Stofko, E., Blumenthal, K., and Salvi, R. (2007) “Proteomic analysis of cisplatin-induced cochlear damage: methods and early changes in protein expression,” Hearing Res., 226, 140-56. [PDF]

Smith, J.J., Cummins, T.R., Alphy, S., and Blumenthal, K.M. (2007) “Molecular interactions of the gating modifier toxin, ProTx-II, with Nav1.5: implied existence of a novel toxin binding site coupled to activation” J. Biol. Chem. 282, 12687-97, doi:10.1074/jbc.M610462200 [PDF]

Smith, J.J., and Blumenthal, K.M. (2007) “Site 3 sea anemone toxins: molecular probes of gating mechanisms in voltage-dependent sodium channels,” Toxicon, 49: 159-70 (invited review). [PDF]

Blumenthal, K.M., Priest, B.T., Smith, A., Smith, J.J., Warren, V.A., and Smith, M.M. (2006) “ProTx-I and ProTx-II: Gating modifiers of NaV and CaV channels,” Toxicon 49: 194-201. [PDF]

Kashlan, O.B., Maaroouf, A.B., Kussius, C., Denshaw, R.M., Blumenthal, K.M, and Kleyman, T.R. (2006) “Distinct structural elements in the first membrane-spanning domain of the epithelial sodium channel,” J. Biol. Chem. 281: 30455-62. doi:10.1074/jbc.M604615200

Lis, M., and Blumenthal, K. (2006) “A modified, dual reporter TOXCAT system for monitoring homodimerization of transmembrane segments of proteins,” Biochem. Biophys. Res. Commun. 339: 321-24.

Smith, J.J, Alphy, S., Seibert, A. L., and Blumenthal, K. M. (2005) Differential Phospholipid Binding by Site 3 and Site 4 Toxins: Implications for Structural Variability Between Voltage-Sensitive Sodium Channel Domains J. Biol. Chem. 280: 11127-11133; 10.1074/jbc.M412552200. [PDF]

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Howell, M.L., Alsabbagh, E., Ma, J.-F., Ochsner, U.A., Klotz, M.A., Beveridge, T.J., Blumenthal, K.M., Niederhoffer, E., Morris, R.E., Dean, G.E., Wani, M, and Hasett, D.J. (2000) AnkB, a Periplasmic Ankyrin-like Protein in Pseudomonas aeruginosa is Required for Optimal Catalase B (KatB) Activity and Resistance to Hydrogen Peroxide. J. Bacteriol. 182, 4545-56.

Michael J. Buck, Ph.D.

Mieczkowski, P.A., Dominska, M., Buck, M.J., Lieb, J.D., and Petes, T.D. (2007).  Loss of a histone deacetylase dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks in Saccharomyces cerevisiae.  PNAS Mar 6;104(10):3955-60.

Xiao, T., Shibata, Y., Rao, B., Laribee, R.N., O’Rourke, R., Buck, M.J., Greenblatt, J.F., Krogan, N.J., Lieb, J.D., Strahl, B.D. (2007).  The RNA Pol II kinase Ctk1 regulates positioning of a 5' histone methylation boundary along genes.  Molecular Cell Biology Jan; 27(2)721-31. 

Buck, M.J., and Lieb, J.D. (2006).  A chromatin-mediated mechanism for specification of conditional transcription factor targets.  Nature Genetics Dec; 38(12): 1446-51. [PDF]

Mieczkowski, P.A., Dominska, M., Buck, M.J., Gerton, J.L., Lieb, J.D., Petes, T.D.  (2006). A global analysis of the relationship between the binding of the Bas1p transcription factor and meiotic recombination activity in Saccharomyces cerevisiae.  Molecular Cell Biology Feb; 26(3): 1014-27.

Buck, M.J., Nobel, A.B., and Lieb, J.D. (2005). ChIPOTle: A user-friendly tool for the analysis of ChIP-chip data.  Genome Biology 6: R97.  [PDF]

Buck, M.J., and Atchley, W.R. (2005). Networks of coevolving sites in structural and functional domains of serpin proteins.  Molecular Biology and Evolution Jul; 22(7):1627-34.

Buck, M.J., and Lieb, J.D. (2004).  ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments. Genomics Mar; 83(3): 349-60. [PDF]

Christine Campbell, Ph.D.

Carlson, H., Ota, S., Campbell, C.E. and Hurlin, P.J. (2001) A dominant repression domain in Tbx3 mediates transcriptional repression and cell immortalization: relevance to mutations in Tbx3 that cause ulnar-mammary syndrome. Hum. Molec. Genet. 10: 2403-2413.

Sinha, S., Abraham, S., Gronostajski, R.M. and Campbell, C.E. (2000) Differential DNA binding and transcription modulation by three T-box proteins T, TBX1 and TBX2. Gene 258: 15-29.

Michael D. Garrick, Ph.D.

Garrick, M.D. and Garrick, L.M. (2007) Loss of rapid transferrin receptor recycling due to a mutation in Sec15l1 in hbd mice. Biochim. Biophysi. Acta – Molec. Cell Res. 1773:105–108.

Ghio, A.J., Turi, J.L., Madden, M.C., Dailey, L.A., Richards, J.D., Stonebuerner, J.G., Morgan, D.L., Singleton, S., Garrick, L.M., Garrick, M.D. (2007) Lung injury after ozone exposure is iron dependent. Am J Physiol Lung Cell Mol Physiol 292: L134-L143.

Garrick, M. D., Kuo, H.C., Vargas, F., Singleton, S., Zhao, L., Smith, J. J., Paradkar, P., Roth, J., Garrick. L. (2006) Comparison of mammalian cell lines expressing distinct isoforms of divalent metal transporter 1 in a tetracyclin-regulated fashion. Biochem. J. (2006) 398, 539-546 (Printed in Great Britian) doi: 10.1042/BJ20051987

Michael, S., Petrocine, S. V., Qian, J., Lamarche, J. B., Knutson, M. D., Garrick, M. D., and Koeppen, A. H. (2006) Iron and iron-responsive proteins in the cardiomyopathy of Friedreich's ataxia. The Cerebellum 5:257-267.

Garrick, M.D., and Garrick, L.M. (2004) Divalent metal transporter DMT1 (SLC11A2) Chapter 3.2 in Membrane Transporter Diseases, pp. 107-122 (ed. by S. Broer and C. Wagner), Kluwer, Dordrecht.

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Garrick, M.D., Núñez, M.T., Olivares, M., and Harris, E.D. (2003) Parallels and Contrasts between Iron and Copper Metabolism. BioMetals 16: 1-8.

Garrick, M.D., Dolan, K.G., Horbinski, C., Ghio, A., Higgins, D., Porubcin, M., Moore, E.G., Hainsworth, L.N., Umbreit, J.N., Conrad, M.E., Feng, L., Lis, A., Roth, J.A., Singleton, S., and Garrick, L.M. (2003) DMT1: A Mammalian Transporter for Multiple Metals. BioMetals 16: 41-54.

Ghio, A.J., Wang, X., Silbajoris, R., Garrick, M.D., Piantadosi, C.A., and Yang, F. DMT1 expression is increased in the lungs of hypotransferrinemic mice. Amer. J. Physiol. Lung Cell. Mol. Physiol. In press.

Garrick, M.D. & Dolan, K.G. (2002) An Expression System for a Transporter of Iron and Other Metals. Chapter 18 in Ultrastructure and Molecular Biology Protocols for Oxidants and Antioxidant, Methods in Molecular Biology, vol. 196, pp. 147-154 (ed. by D. Armstrong), Humana, Totawa.*|

Roth, J.A., Horbinski, C., Higgins, D., Lein, P., and Garrick, M.D. (2002) Mechanism of Manganese-induced Rat Pheochromocytoma (PC12) Cell Death and Cell Differentiation. NeuroToxicology 23: 147-157|.

Marc S. Halfon, Ph.D.

Zhu, Q. and Halfon, M.S. (2009). Complex organizational structure of the genome revealed by genome-wide analysis of single and alternative promoters in Drosophila melanogaster. BMC Genomics, 10:9, doi:10.1186/1471-2164-10-9.

Ivan, A., Halfon, M. S. and Sinha, S. (2008). Computational discovery of cis-regulatory modules in Drosophila without prior knowledge of motifs. Genome Biology, 9:R22. 

Halfon, M. S., Gallo, S. M. and Bergman, C. M. (2008). REDfly 2.0: an integrated database of cis-regulatory modules and transcription factor binding sites in Drosophila. Nucleic Acids Res. 36(Suppl_1):D594-598. doi: 10.1093/nar/gkm876.

Li, L., Zhu, Q., He, X., Sinha, S. and Halfon, M. S. (2007). Large-scale analysis of transcriptional cis-regulatory modules reveals
both common features and distinct subclasses. Genome Biology, 8:R101.

Halfon, M. S. (2006). (Re)modeling the transcriptional enhancer. Nat Genet 38(10): 1102-1103.

Choe, S. E., Boutros, M., Michelson, A. M., Church, G. M. and Halfon, M. S. (2005). Preferred analysis methods for Affymetrix GeneChips revealed by a wholly-defined control dataset. Genome Biology. 6:R16.

Halfon, M. S., Grad, Y., Church, G. and Michelson, A.M. (2002). Computation-based discovery of related transcriptional regulatory modules and motifs using a combinatorial model. Genome Res. 12:1019-1028.

Daniel J. Kosman, Ph.D.

Kwok, E.Y., Severance, S. and Kosman, D.J. (2006) Evidence for iron channeling in the Fet3p, Ftr1p high affinity iron uptake complex in the yeast plasma membrane. Biochemistry, 45:6317-6327. [PDF]

Singh, A., Severance, S., Kaur, N., Wiltsie, W. and Kosman, D. J. (2006) Assembly, activation and trafficking of the Fet3p, Ftr1p high affinity iron permease complex in Saccharomyces cerevisiae. J. Biol. Chem., 281: 13355-13364 [PDF]

Kowk, E. Y., Stoj, C. S., Severance, S., and Kosman, D. J. (2006) An engineered bifunctional high affinity iron uptake protein in the yeast plasma membrane. J. Inorg. Biochem. 100:1053-1060 [PDF]

Taylor, A. B., Stoj, C. S., Ziegler, L., D. J. Kosman and P. J. Hart, (2005) The copper-iron connection in biology: the structure of the yeast metallo-oxidase, Fet3p. Proc. Natl. Acad. Sci. U.S.A. 102:15459-15464 [PDF]

Kwok, E., and Kosman, D. J. (2005) Iron in yeast: mechanisms involved in homeostasis. Top. Curr. Genet. 14, published on-line. [PDF]

Stoj, C. S., and Kosman, D. J. (2005) Copper Oxidases, in Encyclopedia of Bioinorganic Chemistry, 2nd Ed., R. B. King, ed, John Wiley, in press. [PDF]

Quintanar, L., Gebhard, M., *Wang, T.-P., Kosman, D. J., and Solomon, E. I. 2004. Ferrous binding to the multicopper oxidases Saccharomyces cerevisiae Fet3p and human ceruloplasmin: contributions to ferroxidase activity. J. Amer. Chem. Soc. 126:6579-6589.

*Severance, S., *Chakraborty, S., and Kosman, D. J. 2004. The Ftr1p iron permease in the yeast plasma membrane: orientation, topology, and structure-function relationships. Biochem. J. 380:487–496.

Kosman, D. J. (2003) Molecular mechanisms of iron uptake in fungi. Mol. Microbiol. 47:1185-1197 (review article).

Stoj, C., and Kosman, D. J. (2003) Cuprous Oxidase Activity of Yeast Fet3p and Human Ceruloplasmin: Implication for Function. FEBS Lett., 554:422-426. [PDF]

Wang, T.-P., Severance, S., Quintanar, L., Solomon, E. I., and Kosman, D. J. (2003) Targeted suppression of the ferroxidase and iron trafficking activities of the multicopper oxidase, Fet3p, from Saccharomyces cerevisiae. J. Inorg. Biol. Chem. 8:615-622.

Palmer, A. E., Quintanar, L., Severance, S., Wang, T-P., Kosman, D. J., and Solomon, E. I. (2002) Spectroscopic Characterization and O₂ Reactivity of the Trinuclear Cu Cluster of Mutants of the Multicopper Oxidase Fet3p. Biochemistry 41:6438-6448.

Romeo, A. S., Christen, L., Niles, E. G., and Kosman, D. J. (2001) Intracellular chelation of iron by bipyridyl inhibits DNA virus replication: Ribonucleotide Reductase maturation as a probe of intracellular iron pools, J. Biol. Chem. 276:24301-24308.

Machonkin, T. E., Quintanar, L., Palmer, A. E., Hassett, R., Severance, S., Kosman, D. J. and Solomon, E. I. (2001) Spectroscopy and reactivity of the type 1 copper site in Fet3p from Saccharomyces cerevisiae: Correlation of structure with reactivity in the multicopper oxidases. J. Amer. Chem. Soc. 123:5507-5517.

Shiu-Ming Kuo, Ph.D.

Hua P, Tsai W-J, Kuo S-M (2003) Estrogen response element-independent regulation of gene expression by genistein in intestinal cells. Biochim. Biophys Acta 1627: 63-70.

Te-Chung Lee, Ph.D.

Shabbir A, Zisa D, Suzuki G, and Lee T. Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a non-invasive therapeutic regimen. Am J Physiol (Heart Circ Physiol), 2009 in press

Shabbir A, Zisa D, Leiker M, Johnston C, Lin H, and Lee T. Muscular dystrophy therapy by non-autologous mesenchymal stem cells: muscle regeneration without immunosuppression and inflammation. Transplantation, 2009 in press

Missihoun C, Zisa D, Shabbir A, Lin H, and Lee T. Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of d-sarcoglycan-null cardiomyopathy. Mol Cell Biochem 321:45-52, (2009)

Leiker M, Suzuki G, Iyer VS, Canty JM Jr., and Lee T. Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells. Cell Transplantation 17:911-922, (2008)

Lin H, Shabbir A, Molnar M, Yang J, Marion S, Canty JM Jr., and Lee T. Adenoviral expression of vascular endothelial growth factor splice variants differentially regulates bone marrow-derived mesenchymal stem cells. J Cell Physiol 216:458-468, (2008)

Lehning P, Lee T, Fallavollita JA, Canty JM Jr., and Suzuki G. Intracoronary administration of AdvFGF-5 (fibroblast growth factor-5) ameliorates left ventricular dysfunction and prevents myocyte loss in swine with developing collaterals and ischemic cardiomyopathy. Circulation 116:I71-I76, (2007)

Mark R. O'Brian, Ph.D.

Hohle, T.H. and M.R. O’Brian. 2009. The mntH gene encodes the major Mn2+ transporter in Bradyrhizobium japonicum and is regulated by manganese via the Fur protein. Mol. Microbiol. In press.

Small, S.K., S. Puri, I. Sangwan and M.R. O’Brian. 2009. Positive control of ferric siderophore receptor gene expression by the Irr protein in Bradyrhizobium japonicum.  J. Bacteriol. 191: 1361-1368.

Small, S.K., S. Puri and M.R. O’Brian. 2009. Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-Proteobacteria. Biometals, 22: 89-97.

Sangwan, I., S.K. Small and M.R. O’Brian. (2008) The Bradyrhizobium japonicum Irr protein is a transcriptional repressor with high affinity DNA binding activity. J. Bacteriol. 190: 5172-5177.

Amarelle, V., M.R. O’Brian, and E. Fabiano. (2008) ShmR is essential for utilization of heme as a nutritional iron source in Sinorhizobium meliloti. Appl. Envrion. Microbiol. 74: 6473-6475.

Platero, R., Peixoto, L., OBrian, M.R. and Fabiano, E. (2004). Fur is involved in manganese-dependent regulation of mntA (sitA) expression in Sinorhizobium meliloti. Appl. Environ. Microbiol. 70: 4349-4355.

Friedman, Y.E. and OBrian, M.R. (2004) The ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum is an iron-responsive transcriptional repressor in vitro. J. Biol. Chem. 279: 32100-32105.

Friedman, Y.E. and OBrian, M.R. (2003) A novel DNA-binding site for the ferric uptake regulator (Fur) protein from Bradyrhizobium japonicum. J. Biol. Chem. 278: 38395-38401.

Qi, Z. and O'Brian, M.R. (2002) Interaction between the bacterial iron response regulator and ferrochelatase mediates genetic control of heme biosynthesis. Mol. Cell 9: 155-162.

Panek, H. and O'Brian, M.R. (2002) A whole genome view of prokaryotic heme biosynthesis. Microbiol. 148: 2273-2282.

Sangwan, I. and O'Brian, M.R. (2002). Identification of a soybean protein that interacts with GAGA element dinucleotide repeat DNA. Plant Physiol. 129: 1788-1794.

Mulchand S. Patel, Ph.D.

Seifert, F., Golbik, R., Brauer, J., Lilie, H., Schroder-Tittmann, K., Hinze, E., Korotchkina, L. G., Patel, M. S., and Tittmann, K. (2006) Direct Kinetic Evidence for Half-Of-The-Sites Reactivity in the E1 Component of the Human Pyruvate Dehydrogenase Multienzyme Complex through Alternating Sites Cofactor Activition. Biochemistry 45, 12775-12785.

Nemeria, N. S., Korotchkina, L. G., Chakraborty, S., Patel, M. S., and Jordan, F. (2006) Acetylphosphinate is the most potent mechanism-based substrate-like inhibitor of both the human and Escherichia coli pyruvate dehydrogenase components of the pyruvate dehydrogenase complex. Bioorganic Chem. 34, 362-379

Nemeria, N., Chakraborty, S., Baykal, A., Korotchkina, L. G., Patel, M.S., and Jordan, F. (2006) The 1',4'-iminopyrimidine tautomer of thiamin diphosphate is poised for catalysis in asymmetric active centers on enzymes. PNAS 104, 78-82 doi 10.1073/pnas0609973104

Ciszak, E. M., Makal, A., Hong, Y. S., Vettaikorumakankauv, A. K., Korotchkina, L. G. and Patel, M. S. (2006) How dihydrolipoamide dehydrogenase-binding protein binds dihydrolipoamide dehydrogenase in human pyruvate dehydrogenase complex. J. Biol. Chem. 281, 648-655.

Patel, M. S. and Korotchkina, L. G. (2006) Regulation of Pyruvate dehydrogenase complex. Biochem. Soc. Trans. 34, 217-222.

Korotchkina, L. G., Sidhu, S. and Patel, M. S. (2006) Characterization of testes-specific isoenzyme of human pyruvate dehydrogenase. J. Biol. Chem. 281, 9688-9695.

Srinivasan, M., Aalinkeel, R., Song, F., Mitrani, P., Pandya, J. D., Strutt, B., Hill, D. J. and Patel, M. S. (2006) Maternal hyperinsulinemia predisposes rat fetuses for hyperinsulinemia, and adult-onset obesity and maternal mild food restriction reverses this phenotype. Amer. J. Physiol. Endocrinol. 290, 129-134.

Srinivasan, M., Katewa, S. D., Palaniyappan, A., Pandya, J. D. and Patel, M. S. (2006) Maternal high fat diet composition results in fetal malprogramming predisposing to the onset of metabolic-like phenotype in their adulthood. Amer. J. Physiol. Endocrinol. in press.

Ciszak, E.M., Korotchkina, L.G. Dominiak, P.M., Sidhu, S. and Patel, M.S. (2003) Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase. J. Biol. Chem. 278, 21240-21246.

Structure of human pyruvate dehydrogenase (a2b2 structure). The four subunits are arranged tetrahedrally as shown in the following colors: a, red, a', green; b, yellow, and b', blue.

Click on image to enlarge.

Patel, M.S. and Srinivasan, M. (2002) Metabolic Programming: Causes and Consequences (invited minireview). J. Biol. Chem. 277, 1629-1633.

Johnson, M.T., Mahmood, S., Hyatt, S.L., Yang, H.S., Soloway, P.D., Hanson, R.W., Patel, M.S. (2001) Inactivation of the murine phruvate dehydrogenase (Pdha1) Gene and its effect on early embryonic development. Mol. Genet. Metab. 74, 293-302.

Histological analysis of 10.5 days post coitum mouse embryos carrying
the Pdha1Dex8 and normal Pdha1 alleles.  

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Korotchkina, L.G. and Patel, M.S. (2001) Probing the mechanism of inactivation of human pyruvate dehydrogenase by phosphorylation of three sites. J. Biol. Chem. 276, 5731-5738.

Cerebellar cortex: the density of granular cells (G) and
Purkinje cells (P) are reduced in Crebr+ tissue.

Alfred S. Ponticelli, Ph.D.

Dermody J.L., Dreyfuss J.M., Villén J., Ogundipe B., Gygi S.P., Park, P.J., Ponticelli, A.S., Moore, C.L., Buratowski, S. and Buchelli, M.E. (2008). Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PLoS ONE 3: e3273.

Khaperskyy, D.A., Ammerman, M.L., Majovski, R.C. and Ponticelli, A.S. (2008). Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization. Mol. Cell. Biol. 28: 3757-3766.

Fish, R.N., Ammerman, M.L., Davie, J.K., Lu, B.F., Pham, C., Howe, L., Ponticelli, A.S. and Kane, C.M. (2006). Genetic interactions between TFIIF and TFIIS. Genetics 173: 1871-1884

Majovski, R.C., Khaperskyy, D.A., Ghazy, M.A. and Ponticelli, A.S. (2005). A functional role for the switch 2 region of yeast RNAPII in transcription start site utilization and abortive initiation. J. Biol. Chem. 280: 34917-34923

Pal, M., Ponticelli, A.S. and Luse, D.S. (2005). The role of the transcription bubble and TFIIB in promoter clearance by RNA polymerase II. Molecular Cell 19: 101-110.

Ghazy, M.A., Brodie, S.A., Ammerman, M.L., Ziegler, L.M. and Ponticelli, A.S. (2004). Amino acid substitutions in yeast TFIIF confer upstream shifts in transcription initiation and altered interaction with RNA polymerase II. Mol. Cell. Biol. 24: 10975-10985.

Kussius CL, Kaur N, Popescu GK, (2009) Pregnanolone sulfate promites desensitization of activated NMDA receptors, Journal of Neuroscience, (in press).

Zhang, W, Howe, JR, Popescu, GK (2008) Distinct gating modes determine the biphasic relaxation of NMDA receptor currents Nature Neuroscience Oct 26 2008, DOI: 10.1038/nn.2214

Popescu, G (2005) Principles of N-methyl-D-aspartate receptor allosteric modulation. Mol Pharmacol Oct. 68(4):1148-55

Popescu, G (2005) Mechanism-based targeting of NMDA receptor functions. Cell Mol Life Sci Sep. 62(18):2100-11

Popescu, G, Robert A, Howe JR and Auerbach A, (2004) Reaction mechanism determines the NMDA receptor response to repetitive stimulation, Nature 430(7001): 790-793.

Press release; Buffalo Physician article

Popescu, G. and Auerbach A. (2004) The NMDA receptor gating machine: lessons from single-channels. The Neuroscientist 10 (3):192-198

Popescu, G. and Auerbach, A. (2003) Modal gating of NMDA receptors and the shape of their synaptic. Nature Neuroscience 6(5):476-83.

Featured in TINS:  Magleby, KL (2004) Modal gating of NMDA receptors, TINS 27(5), 231-233

Lee Ann Garrett Sinha

John, S.A., Clements, J.L., and Garrett-Sinha, L.A. (2007) Ets-1 regulates plasmacytic differentiation by interfering with the activity of the transcription factor Blimp-1.  Submitted.

Nagaleekar, V.K., Diehl, S., Juncadella, I., Charland, C., Garrett-Sinha, L.A., Muthusamy, N., Anguita, J. and Rincón, M. (2007) Ets1-dependent IP3R3 expression in naïve CD4+ T cell is required for cytokine gene expression.  Submitted.

Clements, J.L., John, S.A., and Garrett-Sinha, L.A. (2006) Impaired generation of CD8+ thymocytes in Ets-1-deficient mice.  J Immunol. 177:905-912.

Wang, D., John, S.A., Clements, J.L., Percy, D.H., Barton, K.P. and Garrett-Sinha, L.A.  (2005) Ets-1 deficiency leads to altered B cell differentiation, hyper-responsiveness to TLR9 and autoimmune disease. International Immunology, 17:1179-1191.

Garrett-Sinha, L.A., Hou, P., Wang, D., Grabiner, B., Araujo, E., Rao, S., Yun, T.J., Clark, E.A., Simon, M.C. and Clark, M.R.  (2005) Spi-1 and Spi-B control the expression of the Grap2 gene in B cells.  Gene, 353:134-146.

Lefebvre, J.M., Haks, M.C., Carleton, M.O., Rhodes, M., Sinnathamby, G., Simon, M.C., Eisenlohr, L.C., Garrett-Sinha, L.A., Wiest, D.L.  (2005) Enforced expression of Spi-B reverses T lineage commitment and blocks b-selection.  J Immunol. 174:6184-6194.

Garrett-Sinha, L.A., Dahl, R., Rao, S., and Simon, M.C.  (2001) PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1.  Blood 97:2908-2912.

Rao, S., Garrett-Sinha, L.A., Yoon, J., and Simon, M.C.  (1999) The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid-restricted heptahelical receptor.  J. Biol. Chem. 274:34245-34252.

Garrett-Sinha, L. A., Su, G. H., Rao, S., Kabak, S., Hao, Z., Clark, M. R., and Simon, M. C.  (1999) PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction.  Immunity 10:399-408.

Su, G. H., Chen, H. M., Muthusamy, N., Garrett-Sinha, L. A., Baunoch, D., Tenen, D. G., and Simon, M. C.  (1997) Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B.  EMBO J. 16:7118-7129.

Click on image to enlarge.

Hu, C-J, Rao, S., Ramirez-Bergeron, D.L., Garrett-Sinha, L.A., Gerondakis, S., Clark, M.R., and Simon, M.C. (2001) PU.1/Spi-B regulation of c-rel is essential for mature B cell survival. Immunity 15:545-555.

Satrajit Sinha, Ph.D.

Romano, R, K, Ortt, B. Birkaya, K. Smalley, and S. Sinha. 2009. An active role of the DN isoform of p63 in regulating basal keratin genes K5 and K14 and directing epidermal cell fate. PLoS ONE in press

Choi, Y.S., R. Chakrabarti, R. Escamilla-Hernandez, and S. Sinha. 2009 Elf5 conditional knockout mice reveal its role as a master regulator in mammary alveolar development: failure of Stat5 activation and functional differentiation in the absence of Elf5. Developmental Biology 329, 227-241. [PDF]

Nagarajan, P., Parikh, N., Garrett-Sinha, LA., Sinha, S. 2009 Ets1 Induces Dysplastic Changes When Expressed in Terminally-Differentiating Squamous Epidermal Cells PLoS ONE. 4(1):e4179.  [PDF]

Parikh, N., Nagarajan, P., Sei-ichi, M., Sinha, S., and Garrett-Sinha, L. A. (2008) Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin. Arch Oral Biol 53: 1091-1100. [PDF]

Landesberg, R., Cozin, M., Cremers, S., Woo, V., Kousteni, S., Sinha, S., Garrett-Sinha, L., Raghavan, S. (2008) Inhibition of oral mucosal cell wound healing by biphosphonates. J. Oral Maxillofac Surg 66, 839-847.

Ortt, K., Raveh, E., Gat, U., Sinha, S. (2008) A chromatin immunoprecipitation screen in mouse keratinocytes reveals Runx1 as a direct transcriptional target of DeltaNp63. J Cell Biochem 104, 1204-19. [PDF]

Nagarajan, P., Romano, R. A and Sinha, S. (2008) Transcriptional control of the differentiation program of interfollicular epidermal keratinocytes. Crit Rev Eukaryot Gene Expr. 18 57-79

Choi, Y.S., Cheng, J., Segre, J., and Sinha, S. (2008) Generation and analysis of Elf5-LacZ mouse: Unique and dynamic expression of Elf5 (ESE-2) in the inner root sheath of cycling hair follicle. Histochem Cell Biol. 129, 85-94 [PDF]


Jennifer A. Surtees, Ph.D.

Lee, S.D.*, Surtees, J.A.*, and Alani, E. (2007) S. cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for mismatch recognition Domain I in DNA repair.  J. Mol. Biol. 366:53-66. [PDF]
* equal authorship

Surtees, J.A. and Alani, E. (2006) Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination.  J. Mol. Biol. 360:523-536. [PDF]

Jiang, J., Bai, L., Surtees, J.A., Gemici, Z., Wang, M.D. and Alani, E. (2005) Detection of high affinity mismatch binding and sliding clamp modes for the MSH2-MSH6 mismatch recognition complex by single-molecule unzipping force analysis.  Mol. Cell.  20:771-781. [PDF]

Surtees, J.A., Argueso, J.L. and Alani, E. (2004) Mismatch repair proteins: Key regulators of genetic recombination.  Cytogenet Genome Res (Special Issue on “Repair Proteins in Meiosis”) 107:146-159.

Surtees, J.A. and Alani, E. (2004) Replication factors license exonuclease I in mismatch repair. Mol. Cell 15:164-166.

MSH proteins recognize and bind misrepairs.
Downstream factors are then recruited to
complete repair.

Mark D. Sutton, Ph.D.

Sutton, M.D., Damage Signals triggering the E. coli SOS response. In: W. Siede, Y.W. Kow, and P.W. Doetsch (Eds.) DNA Damage Recognition. Marcel-Decker, Inc. New York, NY. (Book Chapter). In Press.

Duzen, J.M., Walker, G.C. and Sutton, M.D. (2004) Identification of specific amino acid residues in the E. coli ß processivity clamp involved in interactions with DNA polymerase III, UmuD and UmuD'. DNA Repair 3: 301-312.

Sutton, M. D., Narumi, I., and Walker, G. C. (2002) Posttranslational Modification of the umuD-Encoded Subunit of Escherichia coli DNA Polymerase V Regulates its Interactions with the beta Processivity Clamp. Proc. Natl. Acad. Sci. U.S.A. 99: 5307-5312.

Sutton, M. D., Guzzo, A., Narumi, I., Costanzo, M., Altenbach, C., Ferentz, A. E., Hubbell, W., and Walker, G. C. (2002) A Model for the Structure of the Escherichia coli SOS-Regulated UmuD2 Protein. DNA Repair 1: 77-93.

Sutton, M. D. and Walker, G. C. (2001) Managing DNA Polymerases: Coordinating DNA Replication, DNA Repair, and DNA Recombination. Proc. Natl. Acad. Sci. U.S.A. 98: 8342-8349.

Sutton, M. D., Farrow, M., Burton, B., and Walker, G. C. (2001). Genetic Interactions Between the Escherichia coli umuDC Gene Products and the b Processivity Clamp of the Replicative DNA Polymerase. Journal of Bacteriology 183:2897-2909.

Click on image to enlarge.  

Sutton, M. D. and Walker, G. C. (2001). umuDC-Mediated Cold Sensitivity is a Manifestation of Functions of the UmuD_²C Complex Involved in a DNA Damage Checkpoint Control. Journal of Bacteriology 183:1215-1224.

Sutton, M. D., Murli, S., Opperman, T., Klein, C., and Walker, G. C. (2001). umuDC-dnaQ Interaction and Its Implications for Cell Cycle Regulation and SOS Mutagenesis in Escherichia coli. Journal of Bacteriology 183:1085-1089.

Sutton M. D., Smith, B. T., Godoy, V. G., and Walker, G. C. (2000). The SOS Response: Recent Insights into umuDC-Dependent Mutagenesis and DNA Damage Tolerance. Annual Review of Genetics 34:479-497.

Mary L. Taub, Ph.D.

H.J. Han, M.J. Lim, Y.J. Lee, J.H. Lee, I.S. Yang, and M. Taub, Uric acid inhibits renal proximal tubule cell proliferation via at least two signaling pathways involving PKC, MAPK, cPLA2, and NF-kB, Amer. J. Physiol  (2007) 292: F373-381.

James E. Springate and M. Taub. Ifosfamide Toxicity in Cultured Renal Proximal Tubule Cells, Pediatric Nephrology (2007) 22: 358-365.

M. Borsick, T. Rajkhowa and M. Taub. Evidence for post-transcriptional regulation of Na,K-ATPase by prostaglandin E1. Biochim. Biophys. Res. Com. (2006) 345: 739-745.

K. Matlhagela, and M. Taub. Regulation of the Na,K-ATPase ß1 Subunit Promoter by Multiple Prostaglandin Responsive Elements. Amer. J. Physiol. (2006) 291:F635-F646.

K. Matlhagela, and M. Taub. Involvement of EP1 and EP2 Receptors in the Regulation of the Na,K-ATPase by Prostaglandins in MDCK Cells. Prostaglandins & Other Lipid Mediators (2006) 79: 101-113.

K. Matlhagela and M. Taub. Prostaglandins Regulate Transcription by means of Prostaglandin Response Elements (PGREs) Located in the Promoters Of Mammalian Na,K-ATPase ·1 Subunit Genes. Annals New York Academy of Sciences (2006) 1091: 233-243.

Matlhagela, K., Borsick, M, Rajkhowa, T., and Taub, M. Identification of a prostaglandin-responsive element in the Na,K-ATpase beta 1 promoter that is regulated by cAMP and Ca2+. Evidence for an interactive role of cAMP regulatory element-binding protein and Sp1. J. Biol. Chem. (2005) 280: 334-346.

Gail R. Willsky, Ph.D.

Tracey, A., Willsky, G. R., Takeuchi, E. R. (2007) Vanadium: Chemistry, Biochemistry, Pharmacology and Technical Applications, (Boca Raton FL: CRC Press) 250 pages.

Willsky, Gail R., Godzalla, Michael E. III, Kostyniak, Paul J., Chi, Lai-Har, Gupta, Rohit, Yuen, Violet G., McNeill, John H., Mahroof-Tahir, Mohammad, Smee, Jason J., Yan, Luqin, Lobernick, Aaron, Watson, Shari and Crans, Debbie C., (2007) Comparing Administration Route in Rats with Streptozicin-Induced Diabetes and Inhibition of Myoblast Growth of Vanadium [V(III), V(IV) and V(V)] Dipicolinic Acid Complexes. ACS Symposium Series 974 Vanadium 93-109.

Smee, Jason J., Epps, Jason A., Teissedre, Guillaume, Maes, Mandy, Harding, Nichola, Yang, Luqin, Baruah, Bharat, Miller, Susie M., Anderson, Oren P., Willsky, Gail R., Crans, Debbie C. (2007) 4-Amino- and 4-Nitrodipicolinatovanadium(V) Complexes and Their Hydroxylamido Derivatives: Synthesis, Aqueous, and Solid-State Properties. Inorg. Chem. 46 9827-9840.

Gail R. Willsky1, Lai-Har Chi1, Yulan Liang2, Daniel P. Gaile 2, Zihua Hu3 and Debbie C. Crans (2006) Diabetes Altered Gene Expression in Rat Skeletal Muscle Corrected by Oral Administration of Vanadyl Sulfate Physiol Genomics 26:192-201. [PDF] Supplemental: [PDF]

Hu, Zihua; Willsky, Gail R.. (2006) Utilization of two sample t-test statistics from redundant probe sets to evaluate different probe set algorithms in GeneChip studies.  BMC Bioinformatics 7:12 doi:10.1186/147-2105-7-12 (online journal) [PDF] 

Péter Buglyó, Debbie C. Crans* Eszter M. Nagy, Ruby Lisa Lindo, Luqin Yang, Jason J. Smee,Wenzheng Jin, Lai-Har Chi, Michael E. Godzala III, and Gail R. Willsky* (2005) Aqueous Chemistry of the VanadiumIII and the VIII-Dipicolinate Systems and a Comparison of the Effect of Three Oxidation States of Vanadium Compounds on Diabetic Hyperglycemia in Rats Inorganic Chemistry 44: 5416-5427. [PDF]

Crans, D.C., Smee, J.S., Gaidamauskiene, E.G., Anderson, O.P., Miller, S.M., Jin, W., Gaidamauskas, E., Crubellier, E, Grainda R., Chi, L-H. and Willsky, G.R. (2004) Inhibition of yeast growth by molybdenumhydroxylamine coplexes correlates with their stability in media at differing pH values. J. Inorg. Biochem. 98: 1837-1850.

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