Department of Biochemistry
School of Medicine & Biomedical Sciences
Faculty
MARY L. TAUB, Ph.D.
Professor
Regulation of Kidney Epithelial Cell Growth, Transport and Differentiation
Our laboratory is investigating the molecular mechanisms by which hormones, growth factors and extracellular matrix proteins regulate kidney tubule epithelial cell growth and functional differentiation in vitro. An established canine kidney epithelial cell line, MDCK, and isolated "mutants" are currently being utilized to examine the actions of growth regulatory on the expression of several proteins including the Na+, K+-ATPase and laminin, a glycoprotein in the extracellular matrix. The effects of novel growth regulatory factors on the expression of proteins involved in gluconeogenesis, membrane transport, renal disease and growth control in primary renal cell cultures are being examined. Primary kidney epithelial cells differentiate into nephrons in a reconstituted extracellular matrix proteins is the subject of study.
Selected Recent Publications
H.J. Han, M.J. Lim, Y.J. Lee, J.H. Lee, I.S. Yang, and M. Taub, Uric acid inhibits renal proximal tubule cell proliferation via at least two signaling pathways involving PKC, MAPK, cPLA2, and NF-kB, Amer. J. Physiol (2007) 292: F373-381.
James E. Springate and M. Taub. Ifosfamide Toxicity in Cultured Renal Proximal Tubule Cells, Pediatric Nephrology (2007) 22: 358-365.
M. Borsick, T. Rajkhowa and M. Taub. Evidence for post-transcriptional regulation of Na,K-ATPase by prostaglandin E1. Biochim. Biophys. Res. Com. (2006) 345: 739-745.
K. Matlhagela, and M. Taub. Regulation of the Na,K-ATPase ß1 Subunit Promoter by Multiple Prostaglandin Responsive Elements. Amer. J. Physiol. (2006) 291:F635-F646.
K. Matlhagela, and M. Taub. Involvement of EP1 and EP2 Receptors in the Regulation of the Na,K-ATPase by Prostaglandins in MDCK Cells. Prostaglandins & Other Lipid Mediators (2006) 79: 101-113.
K. Matlhagela and M. Taub. Prostaglandins Regulate Transcription by means of Prostaglandin Response Elements (PGREs) Located in the Promoters Of Mammalian Na,K-ATPase ·1 Subunit Genes. Annals New York Academy of Sciences (2006) 1091: 233-243.
Matlhagela, K., Borsick, M, Rajkhowa, T., and Taub, M. Identification of a prostaglandin-responsive element in the Na,K-ATpase beta 1 promoter that is regulated by cAMP and Ca2+. Evidence for an interactive role of cAMP regulatory element-binding protein and Sp1. J. Biol. Chem. (2005) 280: 334-346.
Han, HJ, Lee, YJ, Park , SH, Lee, JH, and Taub, M. High glucose-induced oxidative stress inhibits Na+/glucose cotransporter activity in renal proximal tubule cells. Amer. J. Physiol. 2005 288: F988-96
Han, HJ, Lee, YJ, Park, JY, Kim, EJ, Lee, JH and Taub, ML Effect of EGF on H2O2 induced inhibition of alpha MG uptake in renal proximal tubule cells: involvement of MAPK and AA release. J. Cell Physiol. 2005 203: 217-225.
Han, , HJ, Sigurdson, WJ, Nickerson, PA and Taub, M (2004) Both mitogen activated protein kinase and the mammalian target of rapamycin modulate the development of functional renal proximal tubules in matrigel. J. Cell Science 2004 117: 1821-1833.
Han, HJ, Park, Y, Jung, J, Lee, Y, Taub, M Epidermal growth factor inhibits 14C-alpha-methyol-D-glucopyranoside uptake in renal proximal tubule cells: involvement of PLC/PKC, p44/42 MAPK and cPLA2. J. Cell Physiol. 2004 199: 206-216.
J.E. Springate and M.L. Taub. Nephrotoxicity of Ifosfamide. Current Topics in Toxicology (2004) 1: 181-184.
M. Taub, M. Borsick, J. Geisel, K. Matlhagela, and C. Allen. (2004) Regulation of the Na,K-ATPase in MDCK Cells by Prostaglandin E1 : A Role for Calcium as well as cAMP. Experimental Cell Research 299: 1-14.H.J. Han, W. Sigurdson, P.A. Nickerson and M. Taub. (2004) Renal Proximal Tubules Develop the Capacity for Transepithelial Solute Transport During the Process of Lumen Formation in a Reconstituted Basement Membrane. J. Cell Science 117: 1821-1833.
E.L Zaki, J.E. Springate and M. Taub. (2003) Comparative Toxicity of Ifosfamide Metabolites and Protective Effect of Mesna and Amifostine in Cultured Renal Tubule Cells. Toxicology In Vitro 17: 397-402.
H.J. Han, Y.H. Lee, S.H. Park and M. Taub (2002) Estradiol-17ß Stimulates Phosphate Uptake and is Mitogenic for Primary Rabbit Renal Proximal Tubule Cells. Experimental Nephrology 10: 355-364.
Taub, M., Han, H.J., Rajkhowa, T., Allen, C., and Park, J.H. (2002) Clonal analysis of immortalized renal proximal tubule cells: Na+/Glucose cotransport system levels are maintained despite a decline in transport function. Experimental Cell Research 281: 205-212.
Park, S.H., Taub, M. and Han, H.J. (2001) Regulation of phosphate uptake in primary cultured rabbit renal proximal tubule cells by glucocorticoids: Evidence for nongenomic as well as genomic mechanisms. Endocrinology 142: 710-720.
