StaffFacultyFaculty Research InterestsPostdoctoral AssociatesGraduate StudentsTechnical Staff
Growth cone motility; intracellular calcium imaging; neuronal growth; imaging of actin dynamics in growth cones; neurotrophic factors in inner ear development.
1. Cohan, C.S., Errick, J.E., and Xia, M-H (1990) Formation of electrical connections between cultured neurons and muscle fibers of the Dev. Brain Res. 64: 167-174.2. Bianchi, L.M. and Cohan, C.S. (1993) Effects of neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factor, Dev. Biol. 159: 353-365.
3. Williams, D.K. and Cohan, C.S. (1994) The role of conditioning factors in the formation of growth cones and neurites from the axon stump after axotomy, Dev. Brain. Res., 81:89-104.
4. Williams, D.K. and Cohan, C.S. (1995) Calcium transients in growth cones of cultured Helisoma neurons in response to conditioning factors, J. Neurobiol. 27: 60-75.
5. Torreano, P. and Cohan, C.S. (1997) Electrically-induced changes in intracellular calcium in Helisoma neurons: regional and neuron-specific differences and implications for neurite outgrowth, J. Neurobiol. 32: 150-162.
6. Welnhofer, E., Zhao, L., and Cohan, C.S. (1997) Actin dynamics and organization during growth cone morphogenesis in Helisoma neurons, Cell Motil. Cytoskel. 37: 54-71.
Development and evaluation of instructional materials including computer-assisted instruction for anatomy.
1. Cotter, J.R. Computer Assisted Instruction for a Medical Histology Course at SUNY at Buffalo: Student Attitudes, Teaching Effectiveness, Time Requirements, and Integration into the Laboratory, Academic Medicine, October, 1997 (in press).2. Cotter J.R, Breen WJ. Histology laboratory exercises by computer: And two more makes eight. In: Kramer P, Sullivan E (eds). Leveraging Learning - Using and Affording Technology. Proceeding of the 5th Conference on Instructional Technologies, 1996 May 21-24, Oswego, NY. State of New York Office of Public Relations, 1996: 45-7.
3. Cotter J.R. CAI in histology: An electronic alternative to a traditional laboratory course of instruction. In: Kramer P (ed). New Faculty Roles In New Learning Environments. Presentations at the 3rd Conference on Instructional Technologies, 1994 June 1-3, Albany, NY. State University of New York Office of Public Relations, 1994: 14-5.
Comparative hematology, effects of low dose X-irradiation upon hemopoisis and erythrocyte morphology/survival/kinetics.
1. Glomski, C.A. and Tamburlin, J., Hard, R., and Chainani, M. (1997) The phylogenetic odyssey of the erythrocyte. IV. The amphibians. Histol. Histopath. 12: 147-170.2. Gong, J.K., Glomski, C.A., and Guo, Y. (1999) A lifelong, wide-range radiation biodosimeter: Erythrocytes with transferrin receptors. Health Physics 77:713-718.
3. Troiano, J.C., Vidal, J.C., Glomski, C., Althaus, R., Gould, E., Scaglione, M., Gould, J., Simoncini, C., and Heker, J. Osmotic fragility and erythrocyte size in captive Crotalus Durissus Terrificus and Bothrops Alternatus (Ophidia-Crotalidae), in press.
Regulation of respiratory cilia; computer analysis of ciliary waveforms; microbiochemical methods; microtubule-based cell motility; optical methods in cell biology, including video-enhanced microscopy and correlative light/electron microscopy.
1. Hard, R., K. Blaustein, L. Scarcello (1992). Reactivation of outer arm depleted axonemes: Evidence for functional differences between inner and outer dynein arms in situ. Cell Motility Cytoskel. 21: 199-209.2. Hard, R., G. Rupp, and M. Withiam (1994). Ciliary coordination in newt lung cells requires microtubule-based linkages between basal bodies. Cell Motility Cytoskel.
3. Hard, R., (1994) Reactivation of respiratory ciliary models. Methods in Cell Biology.
4. Rupp, G. and R. Hard (1994). Outer arm dynein from newt lung respiratory cilia. Purification and polypeptide composition. Cell Motil. Cytoskeleton.
5. Rupp, G. and R. Hard (1994). Reversible phosphorylation modulates beat frequency in respiratory cilia. J. Cell Biol. (Submitted).
6. Park, J.H. and R. Hard (1994). Waveform analysis of reactivated respiratory cilia: Elastic recoil contributes significantly to waveform asymmetry. Cell Motil. Cytoskeleton.
Mechanisms of endothelial locomotion during wound healing and angiogenesis; assembly and regulation of non-muscle myosin in situ; imaging of fluorescent analogs of cytoskeletal proteins in living cells.
1. Kolega, J. 1997. Asymmetry in the distribution of free versus cytoskeletal myosin II in locomoting microcapillary endothelial cells. Exp. Cell Res. 231: 61-82.2. Kolega, J. 1998. Fluorescent analogues of myosin II for tracking the behavior of different myosin II isoforms in living cells. J. Cell Biochem. 68: 389-401.
3. Kolega, J. 1998. Cytoplasmic dynamics of myosin IIA and IIB: spatial "sorting" of isoforms in locomoting cells. J. Cell Sci. 111: 2085-2095.
4. Kolega, J. 1999. Turnover rates at regulatory phosphorylation sites on myosin II in endothelial cells. J. Cell. Biochem. 75: 629-639.
5. Kolega, J., and Kumar, S. 1999. Regulatory light chain phosphorlyation and the assembly of myosin II into the cytoskeleton of microcapillary endothelial cells. Cell Motil. Cytoskel. 43: 255-268.
Adaptive advantages of suspensory behavior; form and function in masticatory apparatus of primitive mammals; intraneural stimulation; functional electrical stimulation, edema control imaging of capillary flow in hamster cheek pouch; effects of electrical fields on endothelial cells.
1. Thamer, S. and Mendel, F.C. Glycol methacrylate preparation for evaluation of intraneural implants in frogs. J. Histotechnology 14(3):175-177, 1991.2. Karnes, J.L., Mendel, F.C., and Fish , D.R. Effects of low voltage pulsed current on edema formation in frog hind limbs following impact injury. Phys. Ther. 72(4): 273-278, 1992.
3. Mendel, F.C., Wylegala, J.A., and Fish, D.R. Influence of high voltage pulsed current on edema formation following impact injury in rats. Phys. Ther. 72(9):668-673, 1992.
4. Mendel, F.C., Caputi, C.D., Karnes, J.L., and Fish, D.R. High voltage pulsed current using surface electrodes: Effect on acute edema formation after hyperflexion injury in frogs. J. Orthop. Sports P.T. 16(3):140-144, 1992.
5. Mendel, F.C. and Fish, D.R. New perspectives in edema control via electrical stimulation. J. Athletic Training 28(1):63-74, 1993.
6. Karnes, J.L., Mendel, F.C., and Fish, D.R., and Burton, H.W. Influence of high voltage pulsed current on diameters of histamine-dilated arterioles. Arch Phys Med Rehab.
Regulation of catecholamine biosynthetic enzymes and growth factors
genes, signal transduction pathways of fibroblast growth factor
receptors, regulation of glial and neuronal development and plasticity
by growth factors, and gene therapy for neurodegenerative disorders.
1. Moffett, Erica Kratz, and Michal K. Stachowiak (1998) Increased tyrosine phosphorylation and novel cis-acting element mediate activation of the fibroblast growth factor-2 (FGF-2) gene by nicotinic acetylcholine receptor. New mechanisms for trans-synaptic regulation of cellular development and plasticity. Molecular Brain Research, 55, 293-305.2. Kim, Hu Peng, F. M. Esparza, S.Z. Maltchenko and M.K. Stachowiak (1998)Cruciform-extruding regulatory element controls cell-specific activity of the tyrosine hydroxylase gene promoter. Nucleic Acid Research, 26, 1793-1800.
3. Moffett J., Kratz E., Stachowiak E.K., Florkiewicz R., Stachowiak M.K. Molecular mechanisms controlling the expression of fibroblastgrowth factor-2 in human astrocytes - implication for cell plasticity. Molecular Biology of the Cell, 9, 1998, 2269-2285.
4. Stachowiak, M.K. Moffett, P. Maher, J. Tucholski, Stachowiak, E.K. Growth factor regulation of cell growth and proliferation in the nervous system - a new intracrine nuclear mechanism. Molecular Neurobiology, 15, 1997, 257 - 285.
5. Horowitz, J.M., Myers, J. Stachowiak, M.K., Torres, G. Identification and distribution of Parkin in rat brain. NeuroReport, 10, 1999, 3393-3397.
Monin, Amber
Matthew, Kevin
Stachowiak, Ewa
Wietchy, Thomas
